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Fig. 6. Effect of recombinant protein GST-4.1R60{Delta}16,18 on microtubule aster formation. The isolated centrosomes were preincubated with buffer (Ct), GST (GST), GST-4.1R80{Delta}16 (GST-4.1R80) or GST-4.1R60{Delta}16,18 (GST-4.1R60) for 45 minutes at 4°C and then tubulin was added to start in-vitro microtubule-aster formation. The microtubule asters stained with anti-{alpha}-tubulin antibody are shown (red). A representative aster (boxed field in the GST-4.1R60 panel) double stained with anti-{alpha}-tubulin (red) and anti-GST (green) antibodies is shown in the enlargements. The recombinant GST-4.1R60 protein is incorporated into the center of the asters and along the microtubules (merge). Four experiments were performed in duplicate. The results are shown in the histogram as the mean ± the standard deviation of the number of microtubule asters assembled in vitro. The images are projections of optical-section stacks acquired by confocal microscopy. Bar, 10 µm.





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