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Fig. 4. PTEN-C/S-GFP does not polarize during chemotaxis. (A) HL60 cells co-expressing PTEN-C/S-GFP and RFP-GPI were analyzed in chemotaxis assays towards fMLP as in Fig. 2A. Dark-phase, green fluorescence, and red and green channel merge are shown; see supplementary material, Movie 4. The asterisk indicates the pipette tip. The cell is representative of 20 out of 22 cells analysed. Bar, 10 µm. (B) Red and green fluorescence scanning of cells in panel A.





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