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Fig. 1. Time course of mRNA expression of fad24 and various adipogenic genes during adipocyte differentiation by northern blot and Q-PCR analyses. (A) Northern blot analysis of fad24 expression. Total RNA from different time points after induction was prepared from 3T3-L1 cells. Isolated total RNA (15 µg) was loaded and subjected to northern blot analysis of fad24. ß-Actin is also shown as a loading control. (B) Q-PCR analysis of fad24 expression. The expression level of fad24 was determined at various time points of adipocyte differentiation of 3T3-L1 cells by Q-PCR and normalized with 18S rRNA expression determined by Q-PCR. Each column represents the mean±s.d. (n=3). (C) Northern blot analysis of expression of various adipogenic genes. Total RNA from various time points after induction was prepared from 3T3-L1 cells. Isolated total RNA (15 µg) was loaded and subjected to northern blot analyses of various adipogenic genes. Staining with ethidium bromide (EtBr) for ribosomal RNA is also shown as a control.
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