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Fig. 4. Tissue distribution of fad24 and the expression profile of fad24 during myogenesis. (A) Distribution of fad24 mRNA in various human tissues. A filter of multiple tissue northern (MTN^TM) blots containing 2 µg of poly(A)⁺ RNA per lane was used to detect the tissue distribution of human fad24 mRNA. ß-Actin was used as a control. A 1.8 kb actin isoform (lower band) is predominant in the heart and skeletal muscle lanes. However, the expression level corresponding to the 2.0 kb band is almost the same, but slightly lower in the heart, skeletal muscle and liver. PBLs, peripheral blood leukocytes. (B) Expression of fad24 in the stromal vascular cells and adipocytes. Epidermal fat pads were isolated from mice. Stromal vascular cells and adipocytes were fractionated and total RNA was isolated. Total RNA (15 µg) was subjected to northern blot analysis, and the expression of fad24 was determined. aP2, whose expression is upregulated in adipocytes, is shown as a control. Staining with EtBr for ribosomal RNA is also shown as a control. (C) Expression of fad24 during differentiation of myoblasts. Total RNA (20 µg) was subjected to northern blot analysis, and the expression of fad24 was determined. The expression of myogenin, whose expression is upregulated in myocytes, is shown as a control. Staining with EtBr for ribosomal RNA is also shown as a loading control.

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