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Fig. 2. Immunodetection of Su(var)3-7 on polytene chromosomes from male and female larvae containing the heat-inducible Su(var)3-7 transgene. (A) Chromosomes after standard fixation. Green, anti-Su(var)3-7; white, DAPI staining. Larvae were kept at 18°C (no hs) or submitted to daily heat-shocks at 35°C (hs). (B) Chromosomes after the same heat-shock conditions, but a milder fixation regime (see Materials and Methods). Arrows indicate the X chromosome.





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