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Fig. 4. Subcellular distribution of MT1-MMP in A375 melanoma cells by immunofluorescence. A375 cells transfected with MT1-MMP and pcDNA3.0 (A-I) or MT1-MMP and furin-GFP (J-L) were plated on coverlips for 16 hours, then fixed and analyzed at the confocal microscope. Cells were double-labeled with immunopurified polyclonal anti-MT1-MMP antibody MMR2 (A,D,G,J) and with antibodies directed against TGN46 (B), transferrin receptor (E) and mannose 6-phosphate receptor (H). Merged staining is also shown (C,F,I,L). Bars, 10 µm.





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