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Fig. 3. Colocalisation of YFP-Rab21 with other CFP-Rab proteins. (A-G) HeLa cells double transfected with YFP-Rab21 wt and other CFP-tagged Rab proteins were fixed and processed for fluorescence microscopy and colocalisation analysis. Examples of Rab21 wt with Rab5a (A-C) and Rab21 wt with Rab11a (D-F) are shown. Arrows indicate colocalising structures, and arrowheads indicate structures containing only Rab21. Bar, 10 µm. Graph in G shows quantitative analysis of colocalisation of discrete Rab21 structures with other Rabs. Error bars represent mean and standard deviation.





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