QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 9. Effect of InsP₃ on atrial EC-coupling. (A) The protocol for activating InsP₃Rs in atrial myocytes. Cells were initially paced under control conditions to monitor the action potential-evoked Ca²⁺ response. A membrane permeant form of InsP₃ (InsP₃BM) was then added to the medium bathing the cells. The arrowheads indicate the points at which cells were depolarised. The filled arrowheads indicate the stimulations that were selected for the analysis shown in Ba-d (i.e. before (Ba) and 2 (Bb), 4 (Bc) or 6 (Bd) minutes after InsP₃BM application). The black and red traces in Ba-d show the response of a subsarcolemmal and central site (the location of the sites is marked on the cell image in Bai). The confocal cell images on the right hand side of Ba-d show the spatial pattern of the Ca²⁺ signals during the experiment. The times at which the images were taken are marked by the correspondingly numbered arrows. The data shown were representative for five cells (from three hearts). (C) Summarises the changes in contraction and in peak Ca²⁺ amplitudes at subsarcolemmal (black circles) and central (red circles) sites during the InsP₃BM application. The data show mean response ± s.e.m. (n=5).

Return to article