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Fig. 10. N-APC mutants weaken kinetochore-microtubule interactions. HCT-116 Myc control and N-APC cells were stained to detect kinetochores (ACA, red), Bub1 or BubR1 (green), microtubules (green or blue) and the chromosomes (red) as indicated. Metaphase cells were then imaged by optical sectioning microscopy. (A,B) Projections of deconvolved image stacks showing that while inter-kinetochore distance is reduced in N-APC-expressing cells, kinetochore bound Bub1 and BubR1 is increased. Scale bars: 5 µm. (C) Bar graph quantifying the distance between sister kinetochores in metaphase cells. Values represent the mean and s.e.m. derived from at least 28 kinetochores in at least 4 cells. (D,E) Bar graphs quantifying the amount of Bub1 and BubR1 staining at metaphase kinetochores. Values represent the mean and s.e.m. derived from at least 68 kinetochores in at least four cells.





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