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Fig. 4. Expression of N-APC accelerates mitotic exit following release from a nocodazole block. Mitotic cells were isolated by selective detachment after a 13-hour nocodazole block, replated into medium containing 0.2 µg/ml nocodazole (solid symbols) or into nocodazole free medium (open symbols), and at the time points indicated, harvested and the mitotic index determined. (A) Line graphs plotting the mitotic index over time, showing that expression of N750 and N1309 accelerates mitotic exit. Values represent the mean and standard error derived from at least two independent experiments, in which at least 2000 cells were counted per time point per experiment. For clarity the data is shown on four separate graphs, with the Myc control data being replicated on each. (B) Bar graph plotting the mitotic index of HeLa, MIN (DLD-1, HCT-116, RKO) and CIN (HT29, LoVo, SW480, SW837) cells 4 hours after release from a 13 hours nocodazole block, showing that with the exception of HT29, CIN cells exit mitosis quicker than MIN cells. Values represent the mean and standard error from three observations with at least 1000 cells being counted.
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