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Fig. 6. N750 survivors contain chromosome aberrations and are highly aneuploid. Following exposure to nocodazole as shown in Fig. 5, metaphase spreads of Myc and N750 cells were prepared at the time points indicated and the chromosomes stained with Hoechst. (A) Histograms plotting the distribution of chromosome numbers at day 0, day 6 and day 36. (B) Examples of two chromosome spreads from N750 cells. Closer inspection revealed that spreads from the N750 cell line contained chromosomes with two constrictions (enlargements). The bar graph quantifies the percentage of metaphases containing chromosomes with two visible constrictions (Myc, n=84; N750, n=77). (C) Anaphase cells at day 6 showing anaphase bridges and lagging chromosomes in N750 cells. The bar graph quantifies the number of cells with abnormal anaphases (Myc, n=26; N750, n=29). (D) Chromosome spreads were prepared from the surviving Myc and N750 cells 36 days after release from 48 hours in 0.02 µg/ml nocodazole. Whereas almost all of the Myc control cells were near diploid, the N750 cells were highly aneuploid.
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