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Fig. 8. Endogenous N-APC localises to the centrosome during the early stages of mitosis. (A) Deconvolved image stacks of DLD-1 cells stained to detect APC (panel I, red in merged image), C-Nap1 (II, green in merged image) and DNA (blue in merged image). IV shows an enlargement of the centrosome boxed in III. V shows a 3D model of the centrosome in IV showing that the N-APC mutant localises to a region between the two centrosomes. (B) Deconvolved image stacks of DLD-1 cells stained to detect APC (red), aurora A (green) and DNA (blue). While N-APC can be detected at the centrosome in prophase and prometaphase (II and III), centrosomal localisation is not apparent in metaphase or anaphase (IV and V). Scale bars: 5 µm.





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