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Fig. 9. Expression of N-APC mutants reduces pole to pole distance. HCT-116 Myc control and N-APC cells were fixed in microtubule stabilising buffer and stained with antibodies against tubulin. Metaphase cells were then imaged by optical sectioning microscopy. (A) Projections of deconvolved image stacks showing representative mitotic spindles. Scale bar: 5 µm. (B) Bar graphs quantifying pole-pole distance. Values represent the mean and s.e.m. derived from at least six cells. (C) Bar graphs quantifying the tubulin density at the spindle midzone. Values represent the mean and s.e.m. derived from at least six cells.





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