QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. TNF ${alpha}$ activates p53 and induces its phosphorylation at serine 18 through p38 kinase. (A) Abundance and status of phosphorylated p53 at serine 18, p53 and p21 in TNF ${alpha}$ -treated MEFs. Serum-arrested MEFs were treated with TNF ${alpha}$ for the indicated time. Total protein lysates were prepared and analysed by gel electrophoresis followed by immuno-blotting analysis using antibodies to p53 phosphorylated at serine 18 (pp53/Ser18), p53 and p21. Each lane contains equal amounts of loaded proteins (40 µg). (B) Abundance and status of phosphorylated p53 at serine 18, p53 and p21 in TNF ${alpha}$ -treated MEFs prealably treated with SB 203580. Serum-arrested cells were pre-treated with SB 203580, followed by a treatment with TNF ${alpha}$ for the indicated time. Lysates were analysed as in (A). (C) Abundance and status of phosphorylated p53 at serine 18, p53 and p21 in TNF ${alpha}$ -treated MEFs expressing Cdc42-N7. Cells were transfected with GFP-tagged Cdc42-N17 before serum-starvation, followed by a treatment with TNF ${alpha}$ for the indicated time. Lysates were analysed as in (A).

Return to article