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Fig. 1. Mutations of AtRabF2b relocalize the chimaera in tobacco leaf epidermal cells. Confocal images 2 days after Agrobacterium inoculation with the indicated mutants at OD₆₀₀ 0.03. (A) YFP-AtRabF2b[wt]. (B) YFP-AtRabF2b[S24N]. (C) GFP-AtRabF2b[Q69L]. (D) GFP-AtRabF2b[wt] (green) and ST-YFP (red). (E) High magnification image of the box in D. Two populations of GFP-AtRabF2b[wt] structures are present: punctate structures that do not colocalize with ST-YFP (arrow) and punctate structures that do (arrowhead). Bar,. (F) GFP-AtRabF2b[S24N] (green), ST-YFP (red). Colocalization is shown in the merged image. (G) GFP-AtRabF2b[Q69L] (green image), ST-YFP (red). GFP-AtRabF2b[Q69L]-positive spherical structures do not colocalize with ST-YFP (arrow, merged image and insert). (H) YFP-AtRabF2b[Q69L] (red) and BobTIP26-1::GFP (green) colocalize with BobTIP26-1::GFP on the tonoplast (arrow, merged image and insert). (I) Cytosolic labelling of YFP-AtRabF2b[N121I]. (J) Cytosolic labelling of YFP-AtRabF2b[C198S,199S]. Bar, 10 µm (A-D and F-J); 5 µm (E); 2 µm (inset G).

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