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Fig. 2. Western blot of AtRabF2b[wt] protein and chimaeras. Proteins were extracted from leaf material at 2 or 4 days after Agrobacterium-mediated transformation. An antibody raised against GFP was used to detect the AtRabF2b fusion protein. (A) Lane 1, protein standard markers; lanes 2-3, AtRabF2b[wt] day 2 and day 4; lanes 4-5, AtRabF2b[S24N] day 2 and day 4; lanes 6-7, AtRabF2b[Q69L] day 2 and day 4; lanes 8-9, AtRabF2b[N123I] day 2 and day 4; lanes 10-11, AtRabF2b[C198,199S] day 2 and day 4; lanes 12-13, PS1-GFP day 2 and day 4; lane 14, untransformed control. (B) Coomassie Blue-stained gel showing equal loading of protein (lanes as above).





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