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Fig. 3. AtRabF2b-tagged chimaeras affect the normal distribution of the pre-vacuolar marker, PS1-GFP. Confocal images of tobacco leaf epidermal cells, 2 days after Agrobacterium inoculation with PS1-GFP (OD₆₀₀ 0.08) and AtRabF2b (OD₆₀₀ 0.03). (A) PS1-GFP (green) and ST-YFP (red). The majority of PS1-GFP does not colocalize with ST-YFP (see inset taken from tangential section of the cell). (B) PS1-GFP (green) and YFP-AtRabF2b[wt] (red) form large aggregates (arrow, merged image). (C) PS1-GFP (green) and YFP-AtRabF2b[S24N] (red) do not form large aggregates (merged image). (D) PS1-GFP (green) and YFP-AtRabF2b[Q69L] (red) form large aggregates (arrow, merged image). (E) PS1-GFP (green) and YFP-AtRabF2b[N123I] (red) do not form large aggregates (merged image). (F) High magnification image of a cell expressing PS1-GFP (green) and YFP-AtRabF2b[wt] (red), showing the nature of the aggregates. (G) High magnification of image in C. Golgi labelling of YFP-AtRabF2b[S24N] colocalizes with a population of PS1-GFP (arrowhead), also present is a population of PS1-GFP that do not colocalize with YFP-AtRabF2b[S24N] (arrow). Bar, 10 µm (A-E); 5 µm (F,G).

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