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Fig. 4. Untagged AtRabF2b chimaeras also affect the normal distribution of the pre-vacuolar marker, PS1-GFP. Confocal images of tobacco leaf epidermal cells, 2 days after Agrobacterium inoculation with PS1-GFP (OD600 0.08) and untagged-AtRabF2b (OD600 0.03). (A) Representative cell transformed with PS1-GFP and untagged AtRabF2b[wt]. Large aggregates are present in the absence of the fluorescent tag (arrow). (B) Representative cell transformed with PS1-GFP and untagged AtRabF2b[Q69L]. Again, large aggregates containing PS1-GFP are present (arrow). Bar, 10 µm.





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