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Fig. 5. AtRabF2b localizes to the aleu-GFP-labelled PVC. Confocal images of tobacco leaf epidermal cells, 2 days after Agrobacterium inoculation with aleu-GFP (OD₆₀₀ 0.1) and AtRabF2b (OD₆₀₀ 0.03). (A) Aleu-GFP faintly fluoresces in the vacuole and also labels small punctate structures. (B) Aleu-GFP (green) and PS1-YFP (red). Aleu-GFP localizes to the PVC compartment also labelled with PS1-YFP (arrow). (C) Aleu-GFP (green), YFP-AtRabF2b[wt]-labelled structures colocalize with PVC-containing aleurain (arrow). Faint YFP-AtRabF2b[wt] structures are still visible (arrowhead). (D) Aleu-GFP (green) and YFP-AtRabF2b[S24N] (red). Bright, punctate aleurain structures do not colocalize with AtRabF2b[S24N] (arrow). (E) Aleu-GFP (green) and YFP-AtRabF2b[Q69L] (red). Aleu-GFP accumulates in AtRabF2b[Q69L] spheres (insert, merged image). Insert image was taken from an additional cell, which clearly showed aleu-GFP accumulating within the spheres. (F) Aleu-GFP (green) and YFP-AtRabF2b[N123I] (red). Aleurain-positive pre-vacuoles are not affected by the presence of YFP-AtRabF2b[N123I]. Bar, 10 µm (A-F); 2 µm in insert (E).

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