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Fig. 8. Location and putative function of AtRabF2b in tobacco leaf epidermal cells. The location of AtRabF2b[wt] on the Golgi apparatus, PVC, and the effect of the dominant-negative mutant on the vacuole targeted protein aleurain-GFP suggest its role in GA to PVC trafficking. The tagged GDP locked mutant AtRabF2b[S24N] localizes entirely to the GA whereas the tagged-GTP locked mutant AtRabF2b[Q69L] localizes to spherical structures at the PVC and also to the tonoplast. (1) Aleu-GFP trafficks through the PVC en route to the vacuole. (2) When untagged AtRAbF2b[S24N] is coexpressed with aleu-GFP, a proportion of the aleu-GFP is secreted to the apoplast. Tagged AtRabF2b[C198,199S] and tagged AtRabF2b[N123I] are entirely cytosolic. aleu-GFP, aleurain-GFP; GA, Golgi apparatus; PVC, prevacuole compartment; V, vacuole.





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