JCS01568 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Expression of GFP-NuMA in HeLa cells. Total cell extracts from HeLa cells transfected with vector only (MOCK) or GFP/NuMA-expressing vector (NuMA-GFP) were separated by size by SDS-PAGE and immunoblotted with NuMA-specific polyclonal antibody. Molecular weight marker indicated is for myosin II.

• Supplemental Figure 2 -

  Fig. S2. Normalized plots of GFP-NuMA recovery after photobleaching in the presence of staurosporine and azide.

• Movie 1 -

  Movie 1. Combined 3D fluorescence (left) and DIC (right) time-lapse recording of an NRK-52E cell expressing NuMA-GFP. This is the same cell as presented in Figs 1,2.

• Movie 2 -

  Movie 2. Slowed down and more highly magnified part of Video 1 that emphasizes intranuclear movements of NuMA-GFP during late prophase.

• Movie 3 -

  Movie 3. Another example of a multi-mode time-lapse recording of NuMA-GFP behavior in NRK-52E cells. This sequence particularly illustrates polewards streaming of NuMA during prometaphase. This is the same cell as presented in Fig. 4.