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Fig. 2. Behavior of GFP-NuMA in NRK52E cells. Selected frames from combinational 3D fluorescence (top half of each frame) / DIC (bottom half of each frame) time-lapse recording of an individual cell. (A) Early prophase. (B) Mid-prophase. (C) Prometaphase. (D) Anaphase onset. (E) Telophase. (F) Cytokinesis. Notice that the distribution of GFP-NuMA closely matches that of endogenous NuMA in the same cell type at all stages of mitosis (Fig. 1). Arrows in (F) indicate doubled NuMA-positive dots that remain visible in most cells during earlier G1. Time in minutes:seconds.





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