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Fig. 3. Intranuclear movements of GFP-NuMA during prophase in NRK 52E cells. (Same cell as in Fig. 2.) This sequence highlights NuMA movements as the cell progresses through late prophase. Selected frames from combinational 3D fluorescence (top half of each frame) / DIC (bottom half of each frame) time-lapse recording of an individual cell. (A-C) Early prophase, (D-E) late prophase. Notice that NuMA aggregates move inside of the nucleus, which still has intact nuclear envelope as revealed by DIC (bottom of each frame). Many NuMA aggregates are seen to move directly towards each other and then fuse (arrows in B,C and D,F). Time in minutes:seconds.





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