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Fig. 4. Polewards transport of GFP-NuMA. Similar to Fig. 3 except that this sequence focuses on NuMA movements after nuclear envelope breakdown (NEB). (A) At NEB, the separated centrosomes (arrows) do not contain appreciable amounts of NuMA. However, immediately after NEB, NuMA begins to `stream' towards the centrosomes (B,C, arrows). (D) Within 2-3 minutes of NEB, most NuMA becomes associated with the forming spindle poles. However, some NuMA aggregates can persist in the peripheral parts of the cell for much longer (A-E, arrowhead). These aggregates gradually decrease in size until they suddenly `stream' toward one of the spindle poles (arrowhead in F-H).





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