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Fig. 5. Dynamic exchange of pole-associated NuMA/GFP revealed by fluorescence recovery after photobleaching. (A-J) Selected frames from combinational time-lapse recording (similar to Figs 2, 3, 4). NuMA associated with one of the two spindle poles during late prometaphase was photobleached (A,B) by a 200 millisecond pulse of 488-nm laser light. The photobleached signal gradually recovered, whereas the intensity of the non-photobleached control pole decreased over time (C-H). Notice that the cytoplasmic double dots of NuMA that are seen in association with the control pole (I,J, arrow) did not appear in the photobleached pole. (K) Normalized intensity of NuMA-GFP fluorescence associated with the irradiated spindle pole over time.





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