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Fig. 8. LGN displaces NuMA from microtubule asters. (top) Indirect immunofluorescence images of microtubule arrangements in untreated extracts or extracts to which LGN has been added (+LGN). The LGN protein was added to samples before microtubule aster assembly (PRE), after microtubule aster assembly (POST) or after microtubule aster assembly along with staurosporine (POST+STR). (bottom) Extracts were separated into 10,000 g soluble (S) and insoluble (P) fractions, separated by size by SDS-PAGE and immunoblotted with antibodies specific to NuMA, Eg5, tubulin and LGN, as indicated. Scale bar, 5 µm.





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