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Fig. 8. Transient over-expression in HeLa cells of EGFP-Rab5Q79L with the RabF or RabSF domains of Rab27a. HeLa cells were transfected with EGFP-Rab5QL/Rab27SF3 (A-I), EGFP-Rab5QL/Rab27F4 (J-L), EGFP-Rab5QL/Rab27SF1-SF4 (M-O) or EGFP-Rab5QL/Rab27SF2 (P-R). After 24 hours, cells were fixed, permeabilised and immunostained with an anti-Tfn-R) antibody (B,K,N,Q), with anti-PDI antibodies (E) or anti-Golgin97 antibodies (H). The panels on the left (A,D,G,J,M,P) are EGFP signal and on the right (C,F,I,L,O,R) are the merged fluorescent signals.





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