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Fig. 6. SNX5 partially colocalizes with the PtdIns(3)P probe, GFP-FYVEEEA1. (A) HeLa cells were transfected with FLAG-SNX5FL and GFP-FYVEEEA1. After 24 hours, cell monolayers were permeabilized and stained with anti-FLAG monoclonal antibody followed by goat anti-mouse IgG-Alexa568 conjugate. (B) HeLa cells transfected with GFP-FYVEEEA1 as above and stained for EEA1 with mouse anti-EEA1 antibodies followed by anti-Ig Alexa568. Confocal images were collected with identical iris settings. Superimposed images (Merge) reveal regions of colocalization. Bar, 10 µm.





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