QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. The PX domain of SNX5 binds to PtdIns(3)P and PtdIns(3,4)P₂. The ability of SNX5_PX domain to bind different phosphoinositides was analysed using a liposome binding assay. ³H-labelled liposomes were prepared as described containing either PtdIns(3)P, PtdIns(3,4)P₂, PtdIns(3,5)P₂, PtdIns(3,4,5)P₃ or no PI, as indicated. Sonicated liposomes (100 µl) were incubated for 30 minutes at room temperature with 20 µg GST or purified GST-SNX5_PX fusion protein immobilized on glutathione-Sepharose beads. After incubation, the beads were washed three times and collected by centrifugation. The radioactivity in both pellets and supernatant was determined. The assay was performed in triplicate and the standard deviations are shown.

Return to article