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Fig. 1. Bi-directional differentiation of E14.5 Dlk+ cells. (A,B) Typical colonies formed from single Dlk+ cells on type IV collagen (A) or laminin (B) after 5 days of culture contain both CK19+ and albumin+ cells. Colonies were stained with anti-CK19 antibody and FITC-conjugated anti-rabbit IgG (green in panels 1), and anti-albumin antibody and Cy3-conjugated anti-goat IgG (red in panels 2). Panels 3 show the merged images of panels 1 and 2. Bar, 100 µm.





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