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Fig. 2. Dlk is significantly downregulated in culture. (A,B) Expression of Dlk was examined in colonies grown on type IV collagen (A, panel 2) and on laminin (B, panel 2) after 5 days of culture. Colonies were incubated with hamster anti-Dlk mAb and biotin-conjugated anti-hamster IgG followed by the treatment with FITC-conjugated streptavidin. The colonies were counterstained with hematoxylin to visualize nuclei (panels 1). Bar, 100 µm. (C) Expression of Dlk was examined by FACS. After 5 days of culture, cells dissociated from type IV collagen-coated plates were incubated with hamster IgG (panel 1) or anti-Dlk mAb (panel 2), and subsequently incubated with FITC-conjugated anti-hamster IgG.





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