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Fig. 6. Effect of translational silencing of p38 ${alpha}$ , p38ß and JNK1/2 on Fas-mediated apoptotic signaling. (A) Dot-plot analysis demonstrates the potentiation of Fas-mediated apoptosis in si-p38ß and si-JNK1/2 cells, and its suppression in si-p38 ${alpha}$ cells compared with that in si-C cells. Apoptotic cells are identified by an increase in annexin-V-FITC positive, PI negative cells (bottom right quadrants). (B) The percentage of cells with decreased ${Delta}$ ${Psi}$ _m (region L) compared with that of cells with high or normal ${Delta}$ ${Psi}$ _m (region H) was enhanced to a greater extent in si-JNK1/2 cells than in si-p38ß cells, but was decreased in si-p38 ${alpha}$ cells. (C and D) Fas-induced cleavage of procaspase-8, procaspase-9 and procaspase-3 was evident in si-C cells and was inhibited in si-p38 ${alpha}$ cell but enhanced in si-p38ß cells (compare lanes 3 and 4 with lane 2, blots 1,2,3, panel C) and in si-JNK cells (compare lanes 3 and 2, panel D). The changes in caspase-9 cleavage correlate with parallel differences in the cytosolic presence of cyt c.

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