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Fig. 7. Fas-induced apoptosis in Jurkat cells is potentiated by ectopically introduced Flag-tagged p38 ${alpha}$ and suppressed by the dominant negative variant p38 ${alpha}$ -AGF. (A) Immunoblot analysis using anti-p38 ${alpha}$ antibody (lanes 1-3) and anti-Flag antibody (lanes 4-6) demonstrate the expression of transfected Flag-p38 ${alpha}$ (lanes 2,5) and Flag-p38 ${alpha}$ -AGF (lanes 3,6). (B) Cells expressing Flag-p38 ${alpha}$ displayed threefold greater sensitivity to Fas-mediated apoptosis than the native Jurkat cells (30.6±1.1 vs 11.4±0.6% apoptotic cells, lanes 3 and 2). By contrast, Flag-p38 ${alpha}$ -AGF expression rendered the cells resistant to Fas-activation (lane 4). (C) The decrease in mitochondrial membrane potential ( ${Delta}$ ${Psi}$ _m) in Jurkat cells (37±2.6%) was increased in cells expressing Flag-p38 ${alpha}$ (66±1.2%) but was suppressed by Flag-p38 ${alpha}$ -AGF (10.7±0.7%). The transfected proteins did not affect the basal levels of apoptosis or ${Delta}$ ${Psi}$ _m (lanes 1,5 and 6).

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