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Fig. 8. DISC composition is altered in p38 and JNK inhibited cells. (A) Lysates of native and Fas-activated cells in the absence and presence of PD169316 and/or SP600125 were immunoprecipitated with a non-agonistic anti-Fas antibody and analyzed for the presence of Fas-associated proteins. The increased presence of FADD in the DISC and the concomitant decrease in the presence of c-FLIPS, but not c-FLIPL, is seen in PD169316-treated native (lane 2) and Fas-activated cells (lane 6) (top panel). Similar changes were observed in presence of SP600125 in the native (lane 3) and Fas-activated (lane 7) cells. These inhibitors did not exert additive effects either in the native (lane 4) or in Fas-activated (lane 8) cells. (B) Presence of c-FLIPS, but not c-FLIPL, in DISC is increased and induced reciprocal changes in DISC-associated FADD and procaspase-8 in si-p38
cells but not in si-p38ß and si-JNK1/2 cells (top panel) in the absence any change in protein in the levels of the all the proteins tested (bottom panel). Data are representative of three separate experiments.
