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Fig. 3. AFM imaging of boar sperm heads with attached particles of DiIC16. (A) Contact mode image to illustrate topographical features and extent of the anterior acrosome, equatorial segment and postacrosome. (B) Line-profile analysis (A-B) across two sperm heads with attached particles (labelled X and Y) on the anterior acrosomal plasma membrane. In each case a small peak, ~200 nm diameter, is coincident with the position of the DiIC16 particle. Bars, 2 µm in A; 1 µm in B.





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