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Fig. 3. Localisation of PH-domain probes during phagocytosis. (A) Time series showing phagocytosis of yeast cells (marked with asterisks) by Dictyostelium amoebae expressing various PH-domain-GFP fusion proteins as indicated. The phosphoinositide binding specificity of the PH domains is indicated. Bars, 5 µm. See also supplementary material Fig. S1a-f. (B) Phagocytosis of 1 µm beads by cells expressing GRP1 and TAPP1-PH-GFP. The brightfield images at t=0 seconds show the localisation of the beads before ingestion, the position of the beads during internalisation is marked by arrows.





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