(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 6. Phagocytosis is impaired in PTEN null cells. (A) The uptake of TRITC-labelled yeast is reduced in PTEN null cells compared with wild-type AX3 cells. Low concentrations of the PI3-kinase inhibitor LY294002 slightly increase yeast uptake while high concentrations reduce phagocytosis of yeast particles. Average and standard deviation of four independent experiments. (B) Localisation of PTEN-GFP during phagocytosis of a yeast cell (asterisks). PTEN-GFP is expressed in PTEN null cells. See also supplementary material Fig. S3. (C) Comparison of GRP1-PH and TAPP1-PH membrane localisation during yeast phagocytosis in wild-type AX2 and PTENnull cells. Curves were aligned with phagosome closure occurring at t=0 seconds (PTENnull/GRP1: four cells averaged; PTENnull/TAPP1: 18 cells).
