|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. The mitotic localization of importin ß is sensitive to temperature-dependent MT depolymerization and regrowth. (A) Monitoring of MT depolymerization and regrowth by staining with 
-tubulin/FITC antibody (a-c). In parallel slides, importin ß was visualized by FITC (d-f) and 
-tubulin by rhodamine (visualized together with DAPI in g-i). Scale bar, 10 µm. (B) Frequency of mitoses in which importin ß did or did not localize at spindle poles during the assay. At least 100 mitoses per condition were analysed (two experiments). (C) Western blot of importin ß in interphase (I) and mitosis (M), with (37°C) or without (0°C) polymerized MTs.
|
