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Fig. 6. Importins {alpha} and ß sediment with polymerized mitotic MTs. (A) Fractions of importin {alpha} and ß sediment with the MT pellet (P) after sucrose centrifugation of mitotic HeLa extracts treated with Taxol, and are enriched in the presence of AMP-PnP, but not with the addition of exogenous MTs. S, supernatant. (B) The incubation of mitotic extract with anti-DIC antibody prevents dynein association with the P fraction. Arrows indicate the position of dynein intermediate chain (DIC), antibody heavy (IgM-h) and light (IgM-l) chains in the S fraction. (C) Western-blot analysis of the indicated proteins in the MT pellet pre-incubated with anti-dynein antibody (anti-DIC lane), non-specific antibody (IgM lane) or buffer alone (BRB80 lane).





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