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Fig. 8. Rescue of importin-ß-dependent mitotic abnormalities by TPX2- and NLS-expressing constructs. (A) Examples of mitotic abnormalities induced by exogenous TPX2 in HeLa cells. Scale bar, 10 µm. (B) Frequency of mitotic abnormalities in HeLa cells transfected with importin ß (pIB) or TPX2 constructs, alone or together (between 150 and 250 mitoses per group were counted in two experiments). Using pGFP vector transfection as the reference group, P<0.01 for TPX2 or pIB alone, and non-significant for TPX2 plus pIB. Using pIB transfection as the reference group, P was non-significant for TPX2 alone and P<0.02 for TPX2 plus pIB. (C) Frequency of mitotic abnormalities in HeLa cells transfected with importin ß (+) or NLS constructs; symbols indicate the molar ratio of NLS to importin-ß sequence used in transfection (between 170 and 260 mitoses per group were counted in two experiments). Using pGFP as the reference group, P<0.001 for pIB; P<0.01 for pIB coexpression with equimolar NLS; P<0.05 or at the limit of significance with higher NLS amount.

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