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Fig. 9. Targeting of importin ß at spindle poles is TPX2 dependent. (A) IF to importin ß (green) in mitotic cells (revealed by DAPI, blue) from cultures treated with siRNA oligonucleotides targeting luciferase (GL2) (top) or TPX2 (bottom); TPX2 is stained in red and images are taken under identical exposure conditions. Scale bar, 10 µm. (B) NuMA recruitment (green) at spindle poles (top) in TPX2-interfered cultures (red); in the lower-magnification field (bottom), NuMA is correctly localized to poles of normal and abnormal spindles. Scale bars, 10 µm. (C) Frequency of mitotic cells showing importin ß (left) or NuMA (right) signals at spindle poles in TPX2- or GL2-interfered cultures (n=150 mitoses per group). (D) Frequency of prometaphases (identified by DAPI staining) showing pole-associated or delocalized importin-ß signals in spindles with normal or abnormal centrosomes, visualized by centrin-2 staining (n=100 mitoses per group).

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