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Fig. 10. Association of sarcomeric protein mRNAs with myofibrillar structures traversing through the nuclear invaginations. Confocal optical sections of FISH detecting sarcomeric protein mRNAs (red) and Alexa 488-phalloidin (green) staining (XY planes) and three-dimensional reconstitutions of the orthogonal planes derived from these sections (XZ and YZ planes). (A) FISH of embryonic fast skeletal muscle MyHC mRNA. Different focal planes (XY1 and 2; XZ1-4) of a myotube are shown. Association of the mRNA with the myofibrillar structures is more evident on the XZ planes. Penetration of myofibrils into the nuclear invaginations is evident on the YZ plane. (B) FISH of skeletal muscle {alpha}-actin mRNA. Different focal planes (XY1 and 2; XZ1 and 2) of a myotube are shown. Arrowheads on the XZ planes indicate association of the mRNA with the myofibrillar structures. Bar, 10 µm.





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