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Fig. 2. Nuclear shape of 10T1/2 myotubes and biochemically differentiated C2C12 cells. (A) Phase-contrast image (a) and corresponding H33258-stained image (b) of a 10T1/2 myotube. 10T1/2 fibroblasts were transfected with MyoD and maintained for 4 days in the differentiation medium. Arrows indicate myotube nuclei with invaginations. Arrowheads indicate smooth nuclei in mononucleated cells. (B) Phase-contrast images (a,c) and corresponding immunofluorescent images detecting muscle-specific MyHC (b,d) of sodium butyrate-treated (a,b) or DMSO-treated (c,d) C2C12 cells. C2C12 myoblasts were cultured for 4 days in the differentiation medium containing either 5 mM sodium butyrate or 2% DMSO. Biochemically differentiated cells were detected by the staining with the mAb MF20 to muscle-specific MyHC. Arrows indicate biochemically differentiated cells with invaginated nuclei. (C) Ratio of smooth-surfaced and grooved or invaginated nuclei. C2C12 Mb, proliferating C2C12 myoblasts. C2C12 Mt, C2C12 myotubes cultured for 4 days in the differentiation medium. C2C12(DMSO), MyHC-positive biochemically differentiated C2C12 cells cultured for 4 days in the differentiation medium containing DMSO. C2Vm8 Mt, C2Vm8 myotubes cultured for 4 days in the differentiation medium. More than 200 nuclei were counted in each experiment. The values are the mean±s.d. of three experiments. Bar, 20 µm.
