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Fig. 8. Formation of the nuclear grooves and invaginations by myofibrillar structures horizontally cutting into the nuclei. (A-C) The nuclei of living C2C12 myotubes expressing EGFP-tagged
-actin were stained with H33342. The fluorescence of EGFP and H33342 was recorded at intervals of 20-30 minutes at 37°C. Shown are selected images of three cells by time-lapse microscopy. The numbers indicate minutes after starting the observation. Arrows point to the positions of nuclei, where myofibrillar structures enter horizontally. Bar, 10 µm.