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Fig. 9. Reduction of straight and longitudinal nuclear grooves and invaginations by preventing myofibrillogenesis. C2C12 myoblasts were cultured for 4 days in the differentiation medium (control) or the medium containing 10 mM BDM or 12 mM KCl to prevent myofibril assembly. (A) Staining of the myotubes with rhodamine-phalloidin (actin), anti-MyHC mAb, and H33258 along with their merged images. (B) Ratio of all the nuclei in myotubes with grooves or invaginations and that of the nuclei with straight and longitudinal grooves or invaginations. More than 200 nuclei were counted in each experiment. The values are mean±s.d. of three experiments. Bar, 10 µm.
