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Fig. 4. Accumulation of NCAM-immunoreactive clusters and associated organelles at the contact site between two neurites. Live hippocampal neurons maintained for three days in culture were labeled with monoclonal NCAM antibodies (red). Intracellular organelles were loaded with FM1-43 applied for 24 hours before the start of recording (green). At the start of recording (0 seconds), the growth cones in the lower right hand corner (arrow) approach the diagonally oriented neurite. NCAM-immunoreactive clusters associated with intracellular organelles marked with FM1-43 and seen in yellow (marked by 1-4) move along the target neurite. During the recording time, three contacts are formed. At the end of the recording (330 seconds), each contact is associated with an NCAM-immunoreactive cluster and intracellular organelles on the target neurite. Bar, 10 µm for all frames. Figure reproduced with permission from The Rockefeller University Press (Sytnyk et al., 2002).
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