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Fig. 4. VEGF stimulates association of ILK with VEGFR complex. (A) VEGF stimulates recruitment of ILK to VEGFR-2. The cells were cultured in the presence or absence of 30 ng/ml of rhVEGF for 5 minutes. The cells were lysed, and anti-VEGFR-2 immunoprecipitates were prepared and immunoblotted with anti-VEGFR-2, anti-phosphotyrosine or anti-ILK antibodies. (B) Inhibition of kinase activity of ILK by LY294002. The cells were stimulated with 30 ng/ml of rhVEGF for 5 minutes, and then ILK activity was measure by ILK kinase assay. LY294002 was added 30 minutes before the VEGF stimulation. Anti-ILK immunoblot was prepared from same immunoprecipitates used for the kinase assay (top panel).





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