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Fig. 5. Effect of ILK-AS on the apoptosis-suppressive activity of VEGF. HUVEC were transiently transfected with the indicated plasmids and incubated for 24 hours. Apoptosis was then induced by serum starvation in the presence or absence of 30 ng/ml of rhVEGF for 48 hours. The cells were then harvested, and apoptotic cell death was determined by flow cytometry analysis using annexin-V-fluorescein. One of two comparable experiments is shown.





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