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Fig. 3. Expression of full-length APC in SW480 cells inhibits cell growth, soft agar colony formation and tumour growth. (A) Cell growth. SW480 and SW480APC.15 cells, seeded in triplicate, were harvested at the time points indicated and counted using a haemocytometer (four fields counted in duplicate). Shown is the mean (±s.d.) number of cells counted from a representative of at least three independent experiments. (i) SW480APC cells (filled symbols) or parental SW480 cells (open symbols) were seeded at either 5x10⁴ cells/ml (squares) or 1x10⁴ cells/ml (diamonds). (ii) SW480 (triangles) and SW480APC (squares) cells were seeded in triplicate at 10⁵ cells/ml. (B) Soft agar colony assay. SW480 or SW480APC.15 cells were seeded in triplicate into 0.3% agar overlayed on 0.6% agar in 35 mm dishes at the densities indicated. Phase contrast micrographs were taken of colonies stained with Crystal Violet formed by SW480 [(i) and (iii)] or SW480APC [(ii) and (iv)] cells after 10 days growth in soft agar. Arrows and arrowheads indicate colonies classified as large and small, respectively. (C) Xenograft tumour growth rate. 5x10⁶ SW480 control cells (clone .7) and 5x10⁶ SW480APC (clone .15) cells were injected into nude Balb/c mice (n=8/group) and tumour volumes of xenografts measured. Shown is the mean (±s.d.) tumour volume (n=8) for SW480 control cells (squares) and SW480APC cells (triangles).

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