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Fig. 5. Reorganization of the actin cytoskeleton in SW480APC cells. Vehicle control-treated SW480 (A-C) or SW480APC.15 (panels D-F), and cytochalasin D-treated SW480 (G-I) or SW480APC.15 (J-L) cells, were co-stained with Rhodamine-Phalloidin to visualize F-actin (A,D,G,J) and antibodies to ß-catenin and Alexa488-conjugated anti-mouse IgG to visualize ß-catenin (B,E,H,K). Areas with overlapping actin (Rhodamine, red) and ß-catenin (Alexa488, green) staining are seen as yellow in C, F, I and L (composite). Fluorescent staining was imaged by laser-scanning confocal microscopy. The images shown are single-representative focal planes. Bar, 10 µm.





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